Fig. 1

Relative gene expression of the virR and revR mutants compared to the wild type. Strand-specific RNA-seq libraries of the wild-type strain JIR325 and its virR and revR mutants were generated from RNA extracted from each strain. Comparative transcriptomic analysis was carried out and differentially expressed genes in the a virR and b revR mutants were identified using the “DESeq” R package. In the MA (log ratios versus mean average) plots that are shown, each point represents a gene. Red dots represent genes with a significant change in expression with a cutoff of FDR > 0.01 and an absolute log₂ fold change > 1. Black dots denote genes with a fold change that is not within the range of the set cutoff. The “Y” axis represents the log₂ fold change compared to the wild type and the “X” axis is the average log₂ normalized counts in two independent biological replicates