Fig. 7

Validation of selected sRNA transcripts. Depth of coverage plots of a SR14, b SR16 c SR42, d SR50 and e SR73 together with their respective genetic organization and RT-PCR results are shown. Depth of coverage plots from the RNA-seq data of the wild-type JIR325 are depicted, where red and green graphs represent transcription on the sense- and antisense-strand, respectively. The Y axes show the coverage depth in reads per million mapped reads (RPM) and the X axes show the corresponding genetic organization. The approximate transcript lengths are indicated in brackets. Whole genes are represented by filled arrows, while open arrows indicate partial genes. RT-PCR was carried out to validate these unannotated transcripts. L, PCR marker ladder; 1, DNA control; 2, wild-type RNA with RT reaction; 3, wild-type RNA with no RT; 4, virR mutant RNA with RT; 5, virR mutant RNA with no RT; 6, revR mutant RNA with RT; 7, revR mutant RNA with no RT. The estimated sizes of the RT-PCR products (in kb) are shown on the left of the agarose gel images