Fig. 4

Phylogeny of Mcam in teleosts following the TGD and SaGD WGDs. Maximum-likelihood phylogeny of Mcam was performed using the PhyML software [38] implemented in the Phylogeny.fr web platform [39] using default “a la carte” parameters and a bootstrapping procedure (N = 100 bootstraps). The resulting tree was exported and edited in Evolview [40]. Input sequences were retrieved using a tblastn search of the PhyloFish database using as bait the zebrafish Mcam protein (XP_005157627.1), in the Mcama branch of the tree. PhyloFish Mcam coding sequences are shown in bold on the tree. The tree was rooted with tetrapod sequences using the Homo sapiens MCAM (AAH56418) and Alligator sinensis Mcam (XP_014373905). A few additional published teleosts Mcam sequences were added in the analysis (normal font). The TGD and SaGD are shown with red stars