Fig. 3
Constitutive and induced defense response conferred by the 2Hb8 QTL to F. graminearum infection. a Genome-wide distribution of DEGs between the 2Hb8 R NIL and M69. The outmost circle represents barley chromosomes 1H-7H. The height of histogram bins represents the number of DEGs in a defined genomic region. Tracks from inside going outside represents DEGs identified in comparisons 2Hb8 R/M69_M96 (yellow), R/M69_M48 (green), R/M69_F96 (blue) and R/M69_F48 (red). b Clustering of DEGs revealed constitutive elevation of defense response in the 2Hb8 R NIL when compared to M69. The 2Hb8 R NIL exhibited increased accumulation of transcripts after mock inoculation at 48 h (M48) and 96 h (M96) or fungal inoculation at 48 h (F48), which accumulated higher in M69 at 96 h (F96). The red color indicates induction and blue color repression. c Venn diagram of up-regulated genes in comparisons of 2Hb8 R/M69_F48, 2Hb8 R/M69_M48, 2Hb8 R/M69_M96 and M69_F96/M96. d A sunburst plot of enriched GO terms identified in up-regulated DEGs in the 2Hb8 R NIL compared to M69 at 48 and 96 hai. Size of individual slices represent statistical significance (−log10FDR). e Categorization of induced (red) or repressed (blue) genes in the 2Hb8 R NIL compared to M69 at 48 and 96 hai