Fig. 2

Reduced 5hmC level at specific brain regions in a mouse model of AD. a-f Representative images of 5hmC dot-blot assay of 12- and 67-week-old WT and DTg mice cortex (a, d. n = 3), cerebellum (b, e. n = 3), and hippocampus (c, f. n = 3). g-i The quantitative results indicated that the global levels of 5hmC did not show significant difference in cortex and cerebellum of WT and DTg mice (g, h). In hippocampus, the overall abundance of 5hmC was significantly decreased in DTg mice compared to WT mice at 67-week stage while it did not show observable difference between WT and DTg mice at 12-week stage (i). (ANOVA post Bonferroni’s Multiple Comparison Test, mean ± s.e.m. *p < 0.05, **p < 0.01)