Fig. 2

Plot of the Anabaena sp. WA102 genome. a The genome is plotted as a black ring with demarcations every 100 kbp. Average GC content in 10 kbp non-overlapping windows is plotted outside of the genome ring. The first track within the genome ring includes the location of the oriC and RNA elements. The oriC was predicted to lie downstream of dnaA among DnaA-binding motifs. The following two interior rings denote predicted protein-coding sequences, first on the positive strand (clockwise) and then on the negative strand (counter-clockwise). NRPS-PKS clusters identified by antiSMASH are shown as red tiles in the fourth interior track. Mobile elements - homing endonucleases and transposases - are plotted on the fifth interior track as orange and yellow tiles, respectively. Contigs from the binned Illumina genome of the culture (Fig. 6) were aligned to the closed genome and 229 gaps in the Illumina assembly are represented as green tiles in the sixth interior track. Green arcs across the center connect repeated regions in the genome, determined by blastn alignment of the finished genome against itself. Note that repeat regions often coincide with gaps in the Illumina assembly. b Genome-wide plot of cumulative GC skew. GC skew was averaged across 1 kbp non-overlapping windows of the genome and then cumulatively summed. Minimum and maximum points on the cumulative GC skew plot should indicate oriC and terC, respectively. However, the signal from the cumulative GC skew is weakened, preventing precise prediction of oriC, terC, and the replicon arms