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Fig. 6 | BMC Genomics

Fig. 6

From: A de novo transcriptome analysis shows that modulation of the JAK-STAT signaling pathway by salmonid alphavirus subtype 3 favors virus replication in macrophage/dendritic-like TO-cells

Fig. 6

Shows a significantly high linear correlation (r2 = 0.8832, P < 0.0001) between RNA-seq and quantitative real time PCR (qRT-PCR) data for the TO-VS-IFN/SAV3 and TO-VS-SAV3. Dotted lines show the 95 % confidence limits for the correlation coefficient (r2) slope (95 % CI 0.8478 - 0.9771). qPCR data was generated as the mean of the fold increase in gene expression relative to the β-actin control group from three independent replicates (N = 3) of TO-cells simultaneously treated with type I IFN and infected with SAV3 (TO-VS-IFN/SAV3) together with none-IFN treated cells infected with SAV3 (TO-VS-SAV3). The cells used in this study were independent of cells used for RNA-seq data analysis

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