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Table 1 Sequencing platforms, library preparation, and sequencing results

From: An optimized protocol for generation and analysis of Ion Proton sequencing reads for RNA-Seq

Sequencer Fragmentation methods Initial RNA(μg) Library name Library insert size(bp) Mean read length(bp) Raw Q10 Raw Q20 Raw Q30 Total reads Total base
Ion Proton Chemical 2 ProC_1 196 153 98.69 % 70.85 % 1.55 % 19,448,438 2,975,809,154
2 ProC_2 179 138 98.70 % 69.81 % 1.34 % 25,262,605 3,505,548,278
ProC_3 200 155 98.82 % 72.14 % 1.80 % 8,139,486 1,263,964,775
2 ProC_4 166 136 98.80 % 72.31 % 1.79 % 23,866,259 3,267,082,715
ProC_5 196 155 98.82 % 73.90 % 1.88 % 15,461,415 2,408,389,343
0.2 ProC_6 175 137 98.74 % 69.79 % 1.35 % 22,673,571 3,123,633,358
ProC_7 215 158 98.75 % 70.67 % 1.43 % 13,098,194 2,076,154,568
0.2 ProC_8 180 140 98.82 % 71.57 % 1.61 % 23,142,854 3,251,986,105
ProC_9 199 160 98.85 % 73.24 % 1.73 % 12,219,286 1,955,402,424
RNaseIII 2 ProR_1 89 94 99.25 % 83.05 % 4.04 % 16,024,785 1,519,618,514
2 ProR_2 91 92 99.17 % 81.95 % 3.87 % 25,434,638 2,364,470,896
HiSeq 2000 Chemical 2 HiSeq_1 193 90;90 98.11 %; 95.11 % 97.03 %; 93.73 % 91.86 %; 88.43 % 60,313,246 10,856,384,280
2 HiSeq_2 187 90;90 97.72 %; 94.53 % 96.59 %; 93.06 % 91.51 %; 87.58 % 56,610,071 10,189,812,780