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Fig. 1 | BMC Genomics

Fig. 1

From: Fluorescence activated cell sorting followed by small RNA sequencing reveals stable microRNA expression during cell cycle progression

Fig. 1

Fluorescence activated cell sorting (FACS) analysis of stained cells and validation of cell cycle sorted populations in HDFa, NCI-H295R and HeLa cells. Panels a-c: FACS analysis shows cell cycle distribution of stained cells before (first image) and after sorting to G1, S and G2 phases (second, third and fourth images, respectively; Panel a: HDFa, Panel b: NCI-H295R, Panel c: HeLa. Intervals of fluorescence intensity (shown as vertical bands) were defined to gate G1, S and G2 phases, respectively. Panels d-e: Western blot analysis confirms the high efficacy of cell cycle sort (Panel d: NCI-H295R, Panel e: HeLa). Density values of phospho(Tyr15)-CDC-2 were first normalized to β-actin loading control and were further normalized to G1 phase (displayed above each band)

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BMC Genomics

ISSN: 1471-2164

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