Table 1 GO term analysis of the cell cycle dependent transcriptional program of HeLa cells
GO Term | Gene count | p-value | Bonferroni-corrected p-value | |
|---|---|---|---|---|
Panel A – genes unique to HeLa SORT experiment | ||||
GO:0007049 | cell cycle | 19 | 4.62 × 10−6 | 3.32 × 10−3 |
GO:0000280 | nuclear division | 10 | 1.99 × 10−5 | 1.42 × 10−2 |
GO:0007067 | mitosis | 10 | 1.99 × 10−5 | 1.42 × 10−2 |
GO:0000087 | M phase of mitotic cell cycle | 10 | 2.29 × 10−5 | 1.64 × 10−2 |
GO:0048285 | organelle fission | 10 | 2.73 × 10−5 | 1.95 × 10−2 |
GO:0051301 | cell division | 11 | 3.41 × 10−5 | 2.43 × 10−2 |
GO:0007017 | microtubule-based process | 10 | 5.94 × 10−5 | 4.19 × 10−2 |
Panel B – genes unique to HeLa synchronization experiment | ||||
GO:0006259 | DNA metabolic process | 15 | 1.20 × 10−8 | 7.35 × 10−6 |
GO:0007049 | cell cycle | 17 | 5.65 × 10−8 | 3.45 × 10−5 |
GO:0006281 | DNA repair | 10 | 2.23 × 10−6 | 1.36 × 10−3 |
GO:0006974 | response to DNA damage stimulus | 11 | 2.59 × 10−6 | 1.58 × 10−3 |
GO:0022403 | cell cycle phase | 11 | 6.53 × 10−6 | 3.99 × 10−3 |
GO:0006260 | DNA replication | 8 | 1.25 × 10−5 | 7.63 × 10−3 |
GO:0033554 | cellular response to stress | 12 | 1.67 × 10−5 | 1.02 × 10−2 |
GO:0000278 | mitotic cell cycle | 10 | 1.89 × 10−5 | 1.15 × 10−2 |
Panel C – overlap of genes of HeLa SORT and synchronization experiments | ||||
GO:0022403 | cell cycle phase | 46 | 2.21 × 10−49 | 1.40 × 10−46 |
GO:0000279 | M phase | 43 | 1.12 × 10−48 | 7.09 × 10−46 |
GO:0000278 | mitotic cell cycle | 44 | 3.69 × 10−48 | 2.33 × 10−45 |
GO:0022402 | cell cycle process | 48 | 2.39 × 10−46 | 1.51 × 10−43 |
GO:0007049 | cell cycle | 52 | 6.85 × 10−46 | 4.33 × 10−43 |
GO:0007067 | mitosis | 37 | 3.87 × 10−45 | 2.45 × 10−42 |
GO:0000280 | nuclear division | 37 | 3.87 × 10−45 | 2.45 × 10−42 |
GO:0000087 | M phase of mitotic cell cycle | 37 | 7.76 × 10−45 | 4.90 × 10−42 |
GO:0048285 | organelle fission | 37 | 1.81 × 10−44 | 1.15 × 10−41 |
GO:0051301 | cell division | 32 | 3.01 × 10−32 | 1.90 × 10−29 |
GO:0007017 | microtubule-based process | 23 | 7.87 × 10−21 | 4.97 × 10−18 |
GO:0000226 | microtubule cytoskeleton organization | 17 | 7.86 × 10−17 | 7.02 × 10−14 |
GO:0007346 | regulation of mitotic cell cycle | 15 | 8.68 × 10−14 | 5.48 × 10−11 |
GO:0051726 | regulation of cell cycle | 19 | 1.68 × 10−13 | 1.06 × 10−10 |
GO:0007051 | spindle organization | 10 | 2.28 × 10−12 | 1.44 × 10-.9 |
GO:0007059 | chromosome segregation | 11 | 2.02 × 10−11 | 1.28 × 10-.8 |
GO:0010564 | regulation of cell cycle process | 12 | 2.90 × 10−11 | 1.84 × 10-.8 |
GO:0007010 | cytoskeleton organization | 18 | 1.70 × 10−10 | 1.08 × 10-.7 |
GO:0051783 | regulation of nuclear division | 9 | 6.98 × 10−10 | 4.41 × 10-.7 |
GO:0007088 | regulation of mitosis | 9 | 6.98 × 10−10 | 4.41 × 10-.7 |
GO:0000070 | mitotic sister chromatid segregation | 8 | 8.88 × 10−10 | 5.61 × 10-.7 |
GO:0000819 | sister chromatid segregation | 8 | 1.09 × 10-.9 | 6.89 × 10-.7 |
GO:0051276 | chromosome organization | 17 | 7.07 × 10-.9 | 4.47 × 10-.6 |
GO:0000075 | cell cycle checkpoint | 9 | 3.58 × 10-.8 | 2.26 × 10-.5 |
GO:0040001 | establishment of mitotic spindle localization | 5 | 6.00 × 10-.8 | 3.79 × 10-.5 |
GO:0051656 | establishment of organelle localization | 8 | 9.92 × 10-.8 | 6.27 × 10-.5 |
GO:0030071 | regulation of mitotic metaphase/anaphase transition | 6 | 1.12 × 10-.7 | 7.07 × 10-.5 |
GO:0007093 | mitotic cell cycle checkpoint | 7 | 1.23 × 10-.7 | 7.76 × 10-.5 |
GO:0051653 | spindle localization | 5 | 1.78 × 10-.7 | 1.13 × 10-.4 |
GO:0051293 | establishment of spindle localization | 5 | 1.78 × 10-.7 | 1.13 × 10-.4 |
GO:0048015 | phosphoinositide-mediated signaling | 8 | 5.37 × 10-.7 | 3.39 × 10-.4 |
GO:0008283 | cell proliferation | 14 | 6.95 × 10-.7 | 4.39 × 10-.4 |
GO:0051640 | organelle localization | 8 | 7.28 × 10-.7 | 4.60 × 10-.4 |
GO:0007052 | mitotic spindle organization | 5 | 1.14 × 10-.6 | 7.17 × 10-.4 |
GO:0051329 | interphase of mitotic cell cycle | 8 | 1.57 × 10-.6 | 9.90 × 10-.4 |
GO:0051325 | interphase | 8 | 1.90 × 10-.6 | 1.20 × 10-.3 |
GO:0007018 | microtubule-based movement | 8 | 2.92 × 10-.6 | 1.85 × 10-.3 |
GO:0000910 | cytokinesis | 6 | 2.93 × 10-.6 | 1.85 × 10-.3 |
GO:0033043 | regulation of organelle organization | 10 | 2.99 × 10-.6 | 1.89 × 10-.3 |
GO:0010948 | negative regulation of cell cycle process | 5 | 1.01 × 10-.5 | 6.36 × 10-.3 |
GO:0007094 | mitotic cell cycle spindle assembly checkpoint | 4 | 2.62 × 10-.5 | 1.64 × 10-.2 |
GO:0045841 | negative regulation of mitotic metaphase/anaphase transition | 4 | 2.62 × 10-.5 | 1.64 × 10-.2 |
GO:0031577 | spindle checkpoint | 4 | 3.47 × 10-.5 | 2.17 × 10-.2 |
GO:0045839 | negative regulation of mitosis | 4 | 3.47 × 10-.5 | 2.17 × 10-.2 |
GO:0051784 | negative regulation of nuclear division | 4 | 3.47 × 10-.5 | 2.17 × 10-.2 |
GO:0051439 | regulation of ubiquitin-protein ligase activity during mitotic cell cycle | 6 | 4.48 × 10-.5 | 2.79 × 10-.2 |
GO:0051438 | regulation of ubiquitin-protein ligase activity | 6 | 7.05 × 10-.5 | 4.36 × 10-.2 |
GO:0051303 | establishment of chromosome localization | 4 | 7.10 × 10-.5 | 4.39 × 10-.2 |
GO:0050000 | chromosome localization | 4 | 7.10 × 10-.5 | 4.39 × 10-.2 |