Fig. 8

Down-regulation of TaAnn10 is associated with the cold-induced male fertility. a Under cold stress (Cold treatment, comprising 10 °C with a 12 h photoperiod), pollen grains from two male sterile lines BS366 and BS400, two recovery lines GLDS and taiyuan806, and BS366 × GLDS and BS366 × Taiyuan806 hybrids lines anthers were devoid of starch (stained with I2-KI). b Rate of seed setting from two male sterile lines BS366 and BS400, two recovery lines GLDS and taiyuan806, and BS366 × GLDS and BS366 × Taiyuan806 hybrids lines. Data presented as means ± SD from three independent experiments, each with fifty plants per line per experiment. c The expression level of TaAnn10 gene in leaf (blue) and anther (red) at meiosis stage under cold treatment (comprising 10 °C with a 12 h photoperiod, sterile conditions), in two male sterile lines BS366 and BS400, two recovery lines GLDS and taiyuan806, and two hybrid lines BS366 × GLDS and BS366 × Taiyuan806. The expression level of wheat actin was used as the internal control to standardize the RNA samples for each reaction. The real-time PCR data are from three biological replicates, and error bars represent the standard error