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Fig. 2 | BMC Genomics

Fig. 2

From: Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing

Fig. 2

Schematic of the ddPCR assay to test the amount of DNA remaining at each step of the library preparation (using DNA fragment specific primers shown by blue arrows [15]) and to measure the amount of DNA fragment bearing adaptors after the ligation step (using the adaptor specific primers shown in orange) and P5/P7 primers after the amplification step (using the P5/P7 primers shown in green)

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BMC Genomics

ISSN: 1471-2164

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