Skip to main content
Fig. 4 | BMC Genomics

Fig. 4

From: Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing

Fig. 4

Bar charts showing the overall DNA library preparation yields of the different tested kits in comparison with the initial DNA input (500 ng). Except where mentioned otherwise all libraries were prepared using the original Illumina Paired end adaptor (also named Sanger adaptors) [6, 22]. After end repair and A-tailing, the DNA loss was estimated using the PhiX specific primers (1st column). After adaptor ligation, both the DNA amount (PhiX primers, 2nd column) and the adaptor ligation efficiency (adaptor specific primers, 3rd column) were measured, except for the Accel kits for which we were not able to measure directly the adaptor ligation efficiency as the adaptor sequences were unknown and are marked by a start (NM for not measured). After PCR, both the total amount of DNA (PhiX primers, 4th column) and the amount of DNA bearing P5 and P7 primers at their ends were measured (P5 & P7 primers, last column)

Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us