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Table 3 Description of the primers and Taqman probe used in ddPCR assay

From: Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing

Oligonucleotide

Sequence

Comments

PhiXa sens

GGC GCT CGT CTT TGG TAT GTA

Amplification and detection of 214 bp fragment

PhiXb sens

TGA ATT GTT CGC GTT TAC CTT

Amplification of 397 bp fragment

PhiXc sens

GTA CGC TGG ACT TTG TAG GAT

Amplification of 568 bp fragment

PhiX rev

GGC GTC CAT CTC GAA G

Amplification and detection of all 3 DNA fragments

Adaptor sens

CTT TCC CTA CAC GAC GCT CTT

Detection of adaptor ligated fragments

Adaptor rev

ATT CCT GCT GAA CCG CTC TTC

Detection of adaptor ligated fragments

P5 primer

AAT GAT ACG GCG ACC ACC GA

Detection of final library fragments

P7 primer

CAA GCA GAA GAC GGC ATA CGA

Detection of final library fragments

Taqman probe

[6FAM]GCGATAACCGGAGTAGTTGAAATG[TAM]

Taqman probe targeting the common sequence between the 3 DNA fragments