Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management

Table 3 Specific primers used for RT-qPCR. Gene ID and main BLAST matches

ID	Gene name	Accession no. of top BLAST hits	Sequence 5′-3′	Product size (bp)	Amplification efficiency (%)
Isotig00959	β-actin	[NP_511052; XP_001986647]	Fwagcacccagtcctcttgac	182	93.0
Isotig00959	β-actin	[NP_511052; XP_001986647]	Rvaacaccatcacccgagtcc	182	93.0
Isotig00208	α 1-elongation factor	[BAM18878; BAG30769]	Fwtgctttcgttcccatctccg	122	90.0
Isotig00208	α 1-elongation factor	[BAM18878; BAG30769]	Rvtcaggcatttaccctcagcc	122	90.0
isotig01415	Odorant binding receptor	[XP_003708550; XP_003494618]	Fwtaaggctcgcaaaggcgaat	182	93.0
isotig01415	Odorant binding receptor	[XP_003708550; XP_003494618]	Rvtgtcgcgtgtgacgatttca	182	93.0
isotig02512	sphingomyelin phosphodiesterase	[XM_001122062; XM_003493337]	Fwccgaggaagggtttccgaat	153	99.0
isotig02512	sphingomyelin phosphodiesterase	[XM_001122062; XM_003493337]	Rvcgttcaggtcggttggtttg	153	99.0
isotig03151	Myrosinasa 1-like	[XP_001601101; XP_001600108]	Fwaggtgcctggaatgtcagtg	205	100.3
isotig03151	Myrosinasa 1-like	[XP_001601101; XP_001600108]	Rvacggtaatggcgaaacccaa	205	100.3
isotig07202	Feminization 1-like	[NP_001153369; EFN87550]	Fwttgttccaggcagctaagca	156	103
isotig07202	Feminization 1-like	[NP_001153369; EFN87550]	Rvgtcacgagtattcaggggca	156	103
isotig06880	Fem 1-like	[EFN89777; EFN60633]	Fwtggttggggaggaaggaaga	239	91.3
isotig06880	Fem 1-like	[EFN89777; EFN60633]	Rvtaaggtcacggtcgcagttg	239	91.3
isotig08283	doublesex	[XP_003700217; ABW99103]	Fwgtcagtaccacagccagctc	152	89.7
isotig08283	doublesex	[XP_003700217; ABW99103]	Rvatgttgtgggactgcggtag	152	89.7

ISSN: 1471-2164