Skip to main content

Table 3 Specific primers used for RT-qPCR. Gene ID and main BLAST matches

From: Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management

ID Gene name Accession no. of top BLAST hits Sequence 5′-3′ Product size (bp) Amplification efficiency (%)
Isotig00959 β-actin [NP_511052; XP_001986647] Fwagcacccagtcctcttgac 182 93.0
Rvaacaccatcacccgagtcc
Isotig00208 α 1-elongation factor [BAM18878; BAG30769] Fwtgctttcgttcccatctccg 122 90.0
Rvtcaggcatttaccctcagcc
isotig01415 Odorant binding receptor [XP_003708550; XP_003494618] Fwtaaggctcgcaaaggcgaat 182 93.0
Rvtgtcgcgtgtgacgatttca
isotig02512 sphingomyelin phosphodiesterase [XM_001122062; XM_003493337] Fwccgaggaagggtttccgaat 153 99.0
Rvcgttcaggtcggttggtttg
isotig03151 Myrosinasa 1-like [XP_001601101; XP_001600108] Fwaggtgcctggaatgtcagtg 205 100.3
Rvacggtaatggcgaaacccaa
isotig07202 Feminization 1-like [NP_001153369; EFN87550] Fwttgttccaggcagctaagca 156 103
Rvgtcacgagtattcaggggca
isotig06880 Fem 1-like [EFN89777; EFN60633] Fwtggttggggaggaaggaaga 239 91.3
Rvtaaggtcacggtcgcagttg
isotig08283 doublesex [XP_003700217; ABW99103] Fwgtcagtaccacagccagctc 152 89.7
Rvatgttgtgggactgcggtag