Fig. 10

Comparison of the dynamics of RNA accumulation of MNSV-Mα5, CMV and WMV in Tendral cotyledons at 3 dpi. a Relative quantification of viral RNA accumulation as a function of time from MNSV, CMV and WMV-infected melon cotyledons. Infected samples at 1 day post inoculation (dpi) were used as calibrators for relative quantification for each virus. Sampling of cotyledons was done until 5 dpi for MNSV and 9 dpi for CMV and WMV. Sampling of second systemic infected leaf was done at 15 dpi for CMV and WMV-infected plants. b RNA accumulation in samples at early stages (1 to 5 dpi) is shown in the insert at a different scale. c RT-qPCR quantification of the accumulation of different genes in MNSV (quantified from 1 to 5 dpi), CMV and WMV (quantified from 1 to 9 dpi) infected plants. Relative accumulation of each gene was calculated in relation to their accumulation in healthy plants for each day of sampling. The values have been log2 transformed