Multiple adaptive routes of Salmonella enterica Typhimurium to biocide and antibiotic exposure

BMC Genomics

Table 1 Susceptibility profiles of Salmonella mutants respect to SL1344 parental strain

N.°	Pre-conditioning agent	Biocide Phenotype	Biocide MIC (mg/L)			Antibiotic MIC (mg/L)							Mutant designation	Fitness Cost (%)	Frequency of mutation
N.°	Pre-conditioning agent	Biocide Phenotype	TRI	BKC	CHX	AMP	CAZ	CIP	ERY	GEN	CLO	TET	Mutant designation	Fitness Cost (%)	Frequency of mutation
	-	Parental strain SL1344	0.06	16	16	1.5	0.38	0.032	32	1.0	3	3
1	NE	TRI^R/BKC^R/CHX^R	2	32	32	1.5	0.38	0.047	32-48	1.5	3	2	NE/TRI1	11	>1.25E-07
2	NE		0.12	32	32	1	0.25	0.032	32	1.0	4	4	NE/CHX2	34	2.92E-09
3	BKC		0.12	32	32	2	0.38	0.023	32	1.5	3	4	BKC/AMP	-	2.50E-09
4	CHX		0.12	32	32	1	0.5	0.032	48	1.5	2	2	CHX/BKC3	-	1.67E-09
5	BKC	TRI^R/BKC^R	0.12	32	16	2	0.38	0.032	48	0.5	4	4	BKC/BKC3	-	2.50E-09
6	CIP	BKC^R	0.06	32	16	1.5	0.5	0.032	24	1.5	2-3	1.5	CIP/TRI1	More fit	<1.50E-05
7	TRI	BKC^R	0.06	32	16	2	0.5	0.032	32	1.5	3	2-3	TRI/BKC3	31	1.50E-09
8	NE	BKC^R/CHX^R/TRI^HS	0.03	32	32	2	0.38	0.032	32-48	1	4	4	NE/BKC2	-	1.25E-07
9	NE		0.03	32	32	1.5	0.75	0.047	24	1.5	3	1.5	NE/BKC3	more fit	2.08E-11
10	TRI		0.015	32	32	1.5	0.5	0.032	24	1.5	2	1.5	TRI/AMP	-	3.06E-09
11	BKC		0.015	64	32	2	0.38	0.023	32	1	3	4	BKC/CHX2	-	1.67E-08
12	CIP	TRI^HS/CHX^HS	0.03	16	8	2	0.38	0.032	32	2	4	3	CIP/CHX1	-	<2.50E-06
13	BKC	TRI^HS	0.015	16	16	1	0.38	0.023	64	1.5	3	1.5	BKC/CIP	-	1.50E-09
14	CHX	TRI^HS	0.015	16	16	2	0.38	0.023	32-48	0.75	3	4	CHX/AMP	17	4.17E-09

NE: non-exposed, - Not done
In the designation of mutants, numbers 1-3 refer to the concentration of compounds in plates as follows: 1- 32 mg/L, 2- 64 mg/L and 3-128 mg/L
Mutants CIP/TRI1 and CIP/CHX1 classified as more fit than SL1344 exhibited -17 % and -21 %, respectively
WGS was performed in the underlined mutants

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