Fig. 4

VDJviz interactive rarefaction plot (diversity vs sampling depth) for T-cell repertoires from two replicate PBMC samples processed with various error correction strategies including quality filtering (q20 and q35 thresholds), elimination of clonotypes encountered only in one of the samples (“intersection”) and frequency-based error correction (“freq”). Solid lines show rarefaction curves computed using observed clonotype frequencies, dashed lines represent their extrapolations. Note that the expected sample diversity is ~90 clonotypes according to UMI-corrected data