Fig. 2

Predicted FA and TAG biosynthetic pathways in perilla seeds. Numbers after the genes (#W) indicate the highest WAF of its expression in developing seeds. Inside of bright green rectangle presents FA biosynthesis and fatty acid export from plastid. Inside of bright red rectangle presents glycerolipids biosynthesis for TAG formation in ER. Yellow circle indicate TAG in oil body. WRI1 transcription factor regulates ɑ-PDH, BCCP, MCMT, KASIII and EAR genes in FA biosynthesis. Red rectangles inside of ER represent acyl-CoA pools and reaction of desaturation by FAD2 and FAD3 in PC, respectively. Acyl-CoA dependent Kennedy pathway is indicated with red arrow. PC-mediated TAG synthesis pathways are indicated with green (by PDAT), blue (by PDCT and CPT) and orange (by LPCAT). Abbreviations: WRI1, wrinkled1; PDHC, plastidial pyruvate dehydrogenase complex; PDH, pyruvate dehydrogenase; DHLAT, dihydrolipoly acyltransferase; LPD, dihydrolipoamide dehydrogenase; ACC, acetyl-CoA carboxylase; CT, carboxyltransferase; BC, biotin carboxylase; BCCP, biotin carboxyl carrier protein; CoA, coenzyme A; ACP, acyl carrier protein; MCMT, malonly-CoA ACP transferase; KAS, ketoacyl-ACP synthase; KAR, 3-ketoacyl-ACP reductase; HAD, 3-hydroxyacyl-ACP dyhydratase; EAR, 2-enoyl-ACP reductase; FAB2, fatty acid biosynthesis2; FATA, acyl-ACP thioesterase A; FATB, acyl-ACP thioesterase B; LACS, long-chain acyl-CoA synthase; FAD2, Δ12 oleic acid desaturase; FAD3, Δ15 (ω-3) linoleic acid desaturase; GPAT9, glycerol 3-phosphate acyltransferase 9; LPAT2, lysophosphatidic acid acyltransferase 2; LPCAT, lysophosphatidylcholine acyltransferase; DGAT, diacylglycerol acyltransferase; PDAT, phospholipid: diacylglycerol acyltransferase; CPT, CDP-choline:DAG cholinephosphotransferase; PDCT, phosphatidylcholinediacylglycerol cholinephosphotransferase; G3P, glycerol-3-phosphate; LPA, lysophosphatidic acid; PA, phosphatidic acid; LPC, lysophosphatidylcholine; PC, phosphatidylcholine; DAG, diacylglycerol; TAG, triacylglycerol; OLN, oleosin