Fig. 4From: RNA sequencing analysis to capture the transcriptome landscape during skin ulceration syndrome progression in sea cucumber Apostichopus japonicus Validation of RNA-seq results using qRT-PCR. Twenty DEGs were selected and their relative fold changes were expressed as the ratio of gene expression in BW of A. japonicus at SUS stages I, II and III compared to H samples as normalized with the Cytb gene. The data obtained from the first RNA-seq and qRT-PCR were compared correspondingly and drawn as a Pearson correlation scatter plotBack to article page