Table 5 Primers used for qRT-PCR validation
Gene | Primer Sequence (5′-3′) |
|---|---|
Cytochrome b | Cytb-F: TGAGCCGCAACAGTAATC |
Cytb-R: AAGGGAAAAGGAAGTGAAAG | |
Complement component 3 | AjC3-F: GCGTTGTTTCGTTCAACAAGGGGA |
AjC3-R: GCCATTCACTGGAGGTGTGCCA | |
Complement factor B | Bf-F: ATTATCTCGCAACAGCGATCC |
Bf-R: GGGCAACCACACCGGCTTCTCCA | |
IRAK4-like | IRAK4-F: TACACGTCAGATCGGGATGA |
IRAK4-R: TAAACGACGAGCGTACCACA | |
NF-kB transcription factor Rel | Rel-F: TGCGAAGCCACATCCATT |
Rel-R: AGGGCATCCTTTAAGTCAGC | |
MAPKKK | MAPKKK -F: GAATCAGAGGAGATAGATGTGGAGA |
MAPKKK -R: AGGAGGAGGAGGAAGACGAC | |
Serine/threonine-protein kinase TBK1-like | TBK1-F: AGATGATGTTGTCCATTCTCG |
TBK1-R: ACAGGAGGAAGTGATGTGCT | |
TGF beta-activated kinase | TAK -F: TCTCTGTAGCCTCCTTTGACG |
TAK -R: CTCGGTCTTCCAACCAACAC | |
BIRC2 | BIRC2-F: TCAGGCACGAGTGACAAAGT |
BIRC2-R: GCATGAGCCATTCACATCTCA | |
NF-kB p105 subunit | p105-F: GCAACACACCCCTCCATCTT |
p105-R: TCTTCTTCGCTAACGTCACACC | |
MyD88 | MyD88-F: CCGATGTAGGAGGATGGTAGTAG |
MyD88-R: CACAGTAAGGTGCTGAAGAATGC | |
HSP 70 | HSP 70-F: AAGAGCACAGGCAAAGAG |
HSP 70-R: TGATGATGGGTTGGCACA | |
HSP 90 | HSP 90-F: TATGAAAGCCTGACAGACGCAAGC |
HSP 90-R: TAACGCAGAGTAAAAGCCAACACC | |
Matrix metalloproteinase-24 | MMP-F: CGATTCAGTCTTCCCTGGTG |
MMP-R: ACCGTCATCAACTTTCCTGGT | |
Apoptosis regulator BAX-like | BAX-F: GCCGTGGGACTGACTTTACA |
BAX-R: TCCATCTCGTAGTTCTCTCAACG | |
Thioredoxin | TRx-F: GCTGGTGACAAACTGGTGAT |
TRx -R: TGAGAAAGACAACGTCGGTA | |
CLECT | CLECT-F: GACGGCTTGTCCAGAGTT |
CLECT-R: AGGTCCATTGTTGGGTTC | |
CLEC19A | CLEC19A-F: ATGCAGCGAGAAGATGGAGT |
CLEC19A-R: TGGCAGGATATGCCCTAGAT | |
GalNAc-specific lectin | GalNAc -F: CCATCCTTCAGGGCAGATAA |
GalNAc -R: TTCATCGACCAAAATGCAGA | |
Major yolk protein | MYP-F: AGGAGGGAGACATTGCTT |
MYP-R: ATGATGCTTTCTGGGTTG | |
PDRG 1 | PDRG1-F: AATTGGAGGAACTCGCTGAA |
PDRG1-R: TTGCTTATCGCCTTCTTGTG |