Discovery Group (n = 74)b | Validation Cohort (n = 54)c | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
CpG-sites | Healthy vs NAFLD&NASH | Fibrosis stage | Steatosis stage | ||||||||
Gene | Total | Sign. | Sign. (%)a | Illumina ID | Coef. | p | p (FDR) | Coef. | p | Coef. | p |
UGT1A1 | 2 | 2 | 100 % | cg19146119 | 4.66E-02 | 8.71E-03 | 1.97E-02 | 8.02E-02 | 4.69E-04 | - | ns |
cg07823755 | -1.34E-02 | 1.18E-02 | 2.57E-02 | -1.26E-02 | 1.41E-02 | - | ns | ||||
UGT1A4 | 5 | 4 | 80 % | cg04437648 | 8.11E-02 | 5.65E-05 | 1.63E-03 | 8.83E-02 | 2.69E-05 | - | ns |
cg05313279 | 5.12E-02 | 5.58E-03 | 1.37E-02 | 8.79E-02 | 1.07E-04 | - | ns | ||||
cg02234120 | -3.48E-02 | 2.15E-04 | 1.93E-03 | -3.02E-02 | 1.69E-03 | - | ns | ||||
cg05141602 | -3.23E-02 | 3.00E-04 | 2.09E-03 | -2.01E-02 | 4.28E-02 | - | ns | ||||
CYP1A2 | 3 | 2 | 67 % | cg11473616 | 6.52E-02 | 6.09E-04 | 2.82E-03 | 7.37E-02 | 3.87E-04 | - | ns |
cg04968473 | 5.22E-02 | 1.54E-02 | 3.22E-02 | 8.93E-02 | 8.76E-04 | - | ns | ||||
SLCO2B1 | 9 | 6 | 67 % | cg12537437 | 7.04E-02 | 9.27E-05 | 1.63E-03 | 8.53E-02 | 3.17E-05 | - | ns |
cg25367084 | 6.36E-02 | 3.09E-04 | 2.09E-03 | 7.40E-02 | 4.99E-05 | - | ns | ||||
cg23577865 | 3.92E-02 | 7.32E-04 | 3.21E-03 | 4.27E-02 | 6.23E-04 | - | ns | ||||
cg20358275 | 3.49E-02 | 6.06E-03 | 1.47E-02 | 4.65E-02 | 5.81E-04 | - | ns | ||||
cg15751948 | 6.04E-02 | 1.06E-04 | 1.64E-03 | 4.73E-02 | 7.48E-03 | - | ns | ||||
cg18589858 | -1.81E-02 | 6.54E-03 | 1.53E-02 | -2.77E-02 | 3.31E-03 | - | ns | ||||
ABCC2 | 5 | 3 | 60 % | cg17044311 | 7.22E-02 | 1.01E-04 | 1.64E-03 | 7.84E-02 | 3.55E-04 | - | ns |
cg14947634 | 7.21E-02 | 2.19E-04 | 1.93E-03 | 7.08E-02 | 9.22E-04 | - | ns | ||||
cg09448875 | 6.81E-02 | 1.87E-04 | 1.88E-03 | 6.60E-02 | 1.74E-03 | - | ns | ||||
CYP2C19 | 6 | 3 | 50 % | cg00051662 | 8.55E-02 | 6.29E-03 | 1.49E-02 | 1.59E-01 | 1.06E-05 | - | ns |
cg16227251 | 6.22E-02 | 5.35E-04 | 2.73E-03 | 7.07E-02 | 6.18E-03 | - | ns | ||||
cg04189838 | 7.22E-02 | 2.41E-02 | 4.95E-02 | 1.71E-01 | 1.02E-05 | - | ns | ||||
UGT2B7 | 2 | 1 | 50 % | cg25583503 | 5.54E-02 | 3.16E-04 | 2.09E-03 | 6.47E-02 | 1.47E-03 | - | ns |
SULT1A1 | 7 | 3 | 43 % | cg01378222 | 6.76E-02 | 2.55E-04 | 1.98E-03 | 1.13E-01 | 1.55E-04 | - | ns |
cg09685060 | 4.34E-02 | 3.10E-03 | 8.53E-03 | 5.18E-02 | 1.14E-04 | - | ns | ||||
cg08008286 | 5.22E-02 | 2.82E-04 | 2.03E-03 | 4.89E-02 | 2.73E-02 | - | ns | ||||
SLC47A1 | 15 | 6 | 40 % | cg08895056 | 3.73E-02 | 3.82E-03 | 1.01E-02 | 7.57E-02 | 2.48E-05 | - | ns |
cg10718608 | 2.85E-02 | 7.89E-03 | 1.80E-02 | 5.52E-02 | 4.48E-04 | - | ns | ||||
cg01530032 | 5.36E-02 | 9.66E-04 | 3.93E-03 | 1.08E-01 | 2.37E-06 | - | ns | ||||
cg12133118 | 3.28E-02 | 1.99E-03 | 6.36E-03 | 4.65E-02 | 4.65E-04 | - | ns | ||||
cg24151087 | 1.52E-02 | 1.86E-02 | 3.84E-02 | 2.03E-02 | 5.65E-03 | - | ns | ||||
cg15014549 | 6.87E-03 | 2.15E-03 | 6.76E-03 | 1.78E-02 | 2.40E-02 | - | ns | ||||
CYP2B6 | 8 | 3 | 38 % | cg19756068 | 5.45E-02 | 1.20E-04 | 1.64E-03 | 4.51E-02 | 3.04E-03 | - | ns |
cg10322876 | 4.75E-02 | 1.38E-03 | 5.10E-03 | 4.99E-02 | 1.79E-03 | - | ns | ||||
cg08852641 | -5.57E-02 | 2.83E-05 | 1.63E-03 | -5.09E-02 | 2.58E-04 | - | ns | ||||
CYP1A1 | 17 | 6 | 35 % | cg12101586 | 6.63E-02 | 1.92E-05 | 1.63E-03 | 1.16E-01 | 1.62E-06 | - | ns |
cg13570656 | 7.61E-02 | 3.93E-05 | 1.63E-03 | 1.45E-01 | 9.83E-06 | - | ns | ||||
cg00213123 | 5.41E-02 | 8.07E-05 | 1.63E-03 | 1.01E-01 | 7.50E-06 | - | ns | ||||
cg26516004 | 7.00E-02 | 8.42E-05 | 1.63E-03 | 1.41E-01 | 1.19E-06 | - | ns | ||||
cg17852385 | 6.24E-02 | 9.05E-05 | 1.63E-03 | 1.47E-01 | 9.82E-06 | - | ns | ||||
cg11924019 | 4.63E-02 | 4.57E-04 | 2.53E-03 | 9.71E-02 | 4.93E-05 | - | ns | ||||
NR2B1 | 9 | 1 | 11 % | cg14651936 | -2.38E-02 | 2.95E-03 | 8.23E-03 | -3.25E-02 | 5.67E-05 | - | ns |
GSTP1 | 10 | 1 | 10 % | cg06928838 | -5.34E-02 | 2.96E-03 | 8.23E-03 | -8.12E-02 | 3.67E-04 | - | ns |