Fig. 1

Binning strategy for RNA-Seq data. Binning protocol shown here is for two theoretical samples, A and B. Schematic on the top shows the different steps in the binning protocol and the outcomes are shown as bar graphs underneath (purple boxes). In the bar graph FPKM units are shown on the y-axis and the gene is represented as a bar in green (sample A) and orange (sample B). Colored lines within the bar represent the constituent isoforms (Yellow boxes). The dashed line represents the threshold (1 FPKM) of gene expression