A next generation sequencing-based method to study the intra-host genetic diversity of norovirus in patients with acute and chronic infection

Table 2 Norovirus reads and coverage per sample

Sample	Reads mapping to NoV	Percentage of NoV reads		Average coverage	% of positions called^a	Length of consensus sequence (bp)
		Vs. non-rRNA reads	Vs. quality filtered reads
OU1	37863	4.01 %	0.51 %	590X	100 %	7532
OU2	88270	5.36 %	1.88 %	1318X	99.55 %	7525
OU3	746	0.04 %	0.01 %	11X	90.59 %	N.A.^b
OU4	1420	2.43 %	0.02 %	22X	N.A.	7657
SP1	102805	8.54 %	1.78 %	1603X	99.91 %	7524
SP2	9589	7.55 %	0.33 %	149X	99.91 %	7535

N.A.: not applicable
^aCoverage was calculated compared to KF509946 (OU1), KC631827 (OU2), KJ196277 (OU3), JN899243.1 (OU4) and KC576909 (SP1 and SP2)
^bThe alignment of OU3 produced 9 non overlapping contigs of 1744, 281, 518, 394, 434, 106, 119, 487 and 2760 nt long

ISSN: 1471-2164