Genomic and expression analyses of Tursiops truncatus T cell receptor gamma (TRG) and alpha/delta (TRA/TRD) loci reveal a similar basic public γδ repertoire in dolphin and human

Table 1 List of primers used in 5′ RACE, RT and genomic PCR

Locus	Primer	Genomic Orient.^a	Sequence 5′–3′	Primer length	Location and sequence positions	Description
TRG	TC3L	REV	TGAGGAGGAGAAGGAGGT	18-mer	TRGC EX3^b 43364–43381	5′RACE, RT-PCR
	TC1L1	REV	GACGATACATACGAGTTCA	19-mer	TRGC EX1^b 37807–37825	dC-TAILED cDNA
	TC1L3	REV	AAGGCAAAGATGTGTTCCAG	20-mer	TRGC EX1^b 37635–37654	nested, RT-PCR
	TC1L2	REV	TGTTGCCATTCTTTTCTTTCC	21-mer	TRGC EX1^b 37692–37712	nested, V1-V2 RT-PCR
	TV1LU	FWD	GCTCGCTCTGACAGTCCTT	19-mer	TRGV1 L-Part1^b 9989–10007	V1 RT-PCR, V1J2 genomic PCR
	TV7LU	FWD	GATCCTCTTCTCCTCCCTCTG	21-mer	TRGV2 L-Part1^b 21785–21805	V2 RT-PCR, V2J3 genomic PCR
	J2GL	REV	TGACGCTCTTGCCATGTGTT	20-mer	TRGJ2^b 31033–31052	V1J2 genomic PCR
	J5BR	REV	CGGCGATGGGACAAAACTTG	20-mer	TRGJ3^b 34698–34717	V2J3 genomic PCR
TRA	TA1C1L	REV	GAAGGTCTGGTTGAAGGTG	19-mer	TRAC EX1^c 86762–86780	5′RACE, RT-PCR
	TA1C2L	REV	TGTCTCCGCATCCCAAATC	19-mer	TRAC EX1^c 86745–86763	dC-TAILED cDNA
	TA1C3L	REV	TGCTGGATTTGGGGCTTCT	19-mer	TRAC EX1^c 86574–86592	nested PCR
TRD	TD1C1L	REV	AGAACTCCTTCACCAGAC	18-mer	TRDC EX1^d 85917–85934	5′RACE, RT-PCR
	TD1C2L	REV	CTTATAGTTACATCTTTGGG	20-mer	TRDC EX1^d 85938–85957	dC-tailed cDNA
	TD2CL	REV	CTGGAGTTTGAGTTTGATT	19-mer	TRDC EX2^d 86773–86791	nested PCR
	VD4U	FDW	GTGGAAGGTTTTGTGGGTCAGG	22-mer	TRDV4 EX^d 91850–91871	V4 genomic PCR
	VD4L	REV	TAACCAAGTGACCCAGATTT	20-mer	TRDV4 EX^d 92062–92081	V4 genomic PCR

^aFWD: forward orientation, REV: reverse orientation (IMGT, Genomic orientation, http://www.imgt.org/IMGTindex/Orientation.php)
^bAcc. Number: JH473572.1
^cAcc. Number: EnsS_112178
^dAcc. Number: JH481615.1

ISSN: 1471-2164