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Fig. 5 | BMC Genomics

Fig. 5

From: Evolutionary and functional analysis of mulberry type III polyketide synthases

Fig. 5

In vivo characterization of CHS and STS by coexpression with 4-coumaroyl-CoA ligase in Escherichia coli. a In vivo assay of CHS. 4-Coumaric acid (1) and naringenin (2) were used as standard compounds. Mn4CL: E. coli-expressed Mn4CL. Mn4CL + MnCHS2: E. coli-coexpressed Mn4CL and MnCHS2. Mn4CL + MnCHS6: E. coli-coexpressed Mn4CL and MnCHS6. Selected ion chromatograms generated during liquid chromatography-electrospray ionization mass spectrometry analyses of the compounds are provided in the small panels on the right: 4-coumaric acid, m/z = 163.1; naringenin, m/z = 271.1. b In vivo assay of STS. Resveratrol (3) was used as the standard compound. Mn4CL: E. coli-expressed Mn4CL. Mn4CL + MnSTS7: E. coli-coexpressed Mn4CL and MnSTS7. Mn4CL + MnSTS8: E. coli-coexpressed Mn4CL and MnSTS8. Selected ion chromatograms of the compound are provided in the small panels on the right: resveratrol, m/z = 227.1

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