Table 1 Primer sequences for used qRT-PCR validation. List of primers and their sequences of selected candidate genes used for qRT-PCR validation of microarray data
GenBank | Gene name | Primer sequence (5′-3′) |
|---|---|---|
Accession number | ||
NM_213770.1 | IRF3: Interferon regulatory factor 3 | F: CCAGTGGTGCCTACACTCCT |
R: AGAGGTGTCTGGCTCAGGAA | ||
NM_001044580 | STAT3 : Signal transducer and activator of transcription 3 (acute-phase response factor) | F: TGCTGGAGGAGAGAATCGT |
R: GGGAATTTGACCAGCAATC | ||
NM_214087 | CD80: Cluster of differentialtion-80 | F: TCAGACACCCAGGTACACCA |
R: GACACATGGCTTCTGCTTGA | ||
NM_001105286.1 | TRAF6: Tumor necrosis factor receptor-associated factor | F: GGGAACGATACGCCTTACAA |
R: CTCTGTCTTAGGGCGTCCAG | ||
NM_213779 | CCL4 : Chemokine (C-C motif) ligand 4 | F: CTCTCCTCCAGCAAGACCAT |
R: CAGAGGCTGCTGGTCTCATA | ||
HQ013301 | GAPDH : Glyceraldehyde-3-phosphate dehydrogenasea | F: GCTGGTGCTGAGTATGTCGT |
R: AAGCAGTTGGTGGTACAGG | ||
XM_003124280.3 | ACTB: Actin, Betaa | F: AAGGACCTCTACGCCAACAC |
R: CTGGCTGATCCACATCTGCT |