Skip to main content
Fig. 5 | BMC Genomics

Fig. 5

From: Genomic and functional analyses unveil the response to hyphal wall stress in Candida albicans cells lacking β(1,3)-glucan remodeling

Fig. 5

Genetic interactions of CHS3, CHS2, CHS8, CHS2/CHS8 and PHR1 during filamentation on solid M199 at pH 7.5 and pH 8. Blastospores were plated as single cells on solid M199-150 mM HEPES buffered at the indicated pH supplemented with uridine 100 μg/ml, and incubated at 37 °C (n = 4). a Colonies produced after 24 hours of incubation at pH 7.5 b Inclusion of sorbitol as an osmotic stabilizer does not suppress lethality of phr1Δ chs3Δ (left panel) whereas the double mutant exhibits normal growth at pH 4.5, a permissive pH (n = 4). c Colonies produced on solid M199-150 mM HEPES pH 8 incubated for 3 days at 37 °C (n = 4). d Microscopic morphology of cells taken from the indicated colonies on plate at pH 8. The scale bar is 20 μm

Back to article page