Fig. 4

The annotation of the early Platynereis transcriptome. a Platynereis gene models with predicted open reading frames (ORFs) were aligned to the Swiss-Prot databases. >70 % of the Platynereis predicted ORFs aligned to vertebrate species. The top five species that the Platynereis predicted ORFs aligned to are Homo sapiens (26 %), Mus musculus (19 %), Drosophila melanogaster (7 %) Rattus norvegicus (6 %), and Bos taurus (5 %). b The Platynereis transcripts were further functionally annotated using Pfam and Swiss-Prot, to describe potential protein domains, and KEGG to identify pathways, and 9642 genes and 17,498 transcripts are shared by all three categories. 28,326 transcripts and 15,690 genes are associated with both Swiss-Prot and Pfam databases, which are 55 and 56 % of the transcript and gene models with predicted ORFs, respectively. c Orthologous analysis was performed using OrthoMCL and the transcriptome data from 18 species including our early Platynereis models was collected. Our Platynereis models were compared with the EMBL Platynereis transcriptome draft version 1.90 which is based on later stages of RNA-seq data. 8024 gene models are supported by all three analyses. (see Results and Methods for details)