Fig. 2

SRM validation of differential protein expression. Peptides corresponding to the indicated proteins (shown here) were quantified using SRM for individual samples in each of control (n = 10), 0.5 mg/kg BW MeHg (n = 9) and 2 mg/kg BW MeHg (n = 9) groups. a) T23O; VFVDLFNLATFLIPR. b) GLNA; RPSANCDPYAVTEALIR. c) EPS8L2; DVQILNCALDDIELLVAR. d) APOA4; LTVNTQDLQSQLAELWK. e) RAP1B; LQIWDTAGQER. Differences between control and treated groups were compared using Student's t test (*p < 0.05, **p < 0.01, and ***p < 0.001). Data are presented as mean ± standard deviation, and the y-axis represents the ratio of intensities between the endogenous peptide (L) and the corresponding isotopically labeled peptide that was added to the sample (H)