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Table 1 Immunolabeling criteria for cell types and meiotic substages

From: Regulatory complexity revealed by integrated cytological and RNA-seq analyses of meiotic substages in mouse spermatocytes

Substages SYCP3 γH2AX STRA8 H1T
Spermatogoniaa None Negative Positive Negative
Preleptonema Patches Negative Positive Negative
Early Leptonema Patches Positive Positive Negative
Late Leptonema Fine foci Positive Weakly positive Negative
Zygonema Partially paired Patches Negative Negative
Early Pachynema Paired Restricted to XY body Negative Negative
Late Pachynema Paired Restricted to XY body Negative Positive
Diplonema Partially dissociated Restricted to XY body Negative Positive
  1. aSpermatogonia are a separate mitotic stage, not a meiotic substage