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Table 1 Immunolabeling criteria for cell types and meiotic substages

From: Regulatory complexity revealed by integrated cytological and RNA-seq analyses of meiotic substages in mouse spermatocytes

Substages

SYCP3

γH2AX

STRA8

H1T

Spermatogoniaa

None

Negative

Positive

Negative

Preleptonema

Patches

Negative

Positive

Negative

Early Leptonema

Patches

Positive

Positive

Negative

Late Leptonema

Fine foci

Positive

Weakly positive

Negative

Zygonema

Partially paired

Patches

Negative

Negative

Early Pachynema

Paired

Restricted to XY body

Negative

Negative

Late Pachynema

Paired

Restricted to XY body

Negative

Positive

Diplonema

Partially dissociated

Restricted to XY body

Negative

Positive

  1. aSpermatogonia are a separate mitotic stage, not a meiotic substage