Fig. 2From: Canalization of gene expression is a major signature of regulatory cold adaptation in temperate Drosophila melanogaster Transcriptome overview: PCA. PCA was calculated using the built-in methods provided by DESeq2 [42] for variance stabilizing transformation of read counts and PCA on the 500 genes with the highest overall expression variance. Note that samples are clearly separated according to continent and condition with the exception of RT and CS samples, which cluster tightly together in both populations such that symbols partly overlapBack to article page