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Fig. 1 | BMC Genomics

Fig. 1

From: A simplified method for identifying early CRISPR-induced indels in zebrafish embryos using High Resolution Melting analysis

Fig. 1

Genomic DNA is suitable for HRM-based genotyping. a Protocol timeline for sample preparation. Genomic DNA extraction, two-steps Evagreen PCR and HRM-based analysis. b Genotyping of 21 adult zebrafish obtained by an incross of glra4a +/− parents. The HRM curve analysis discriminates wild-type (WT), heterozygous (HT) and homozygous (HM) fish. c Sequencing results from each profile identified in (B) confirmed the genotyping and the presence of the 22 base-pair deletion in the heterozygous and homozygous population

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BMC Genomics

ISSN: 1471-2164

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