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Fig. 1 | BMC Genomics

Fig. 1

From: Comparison of pre-processing methods for multiplex bead-based immunoassays

Fig. 1

Examples for a good, a fair and a poor mean-standard deviation, Volcano and Bland-Altman plot. Mean-SD plot: orange line: loess curve; good: scatterplot parallel to x-axis with low variation, sd stable over mean signal intensities; fair: no trend to larger sd for larger means but variation around loess curve; poor: trend. Bland-Altman plot: blue line: zero line; green line: mean difference; orange lines: 95 % CI; good: mean of differences close to zero with small, constant variation around this mean; fair: neither trend nor funnel but mean difference deviating widely from zero or increased scatter; poor: visible trend or funnel. Volcano plot: orange line: loess curve; good: visible funnel, both sides with similar length; fair: one side of the funnel considerably shorter than the other; poor: no funnel shape

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