Table 1 Gene name, accession number, functional pathway, primer sequences, and product length of qPCR validated genes
From: Transcriptome profiling of the rumen epithelium of beef cattle differing in residual feed intake
Gene name (symbol) | GenBank accession number | Functional pathway | Forward and reverse primer sequence (5'-3') | Product length (bp) | Reference |
|---|---|---|---|---|---|
Triosephosphate Isomerase 1 (TPI1) | NM_001013589 | Glycolysis, Gluconeogenesis, Acetylation | Fwd: GGGAGGAAGAACAATCTGGGG | 107 | This study |
Rev: GCGAAGTCAATGTAGGCGGT | |||||
Trans-2,3-Enoyl-CoA Reductase (TECR) | NM_001034748 | Biosynthesis of unsaturated fatty acids, Acetylation | Fwd: CCAAGGGCAAGTCCCTGAAG | 82 | This study |
Rev: AGGTCCCGGAAGTAGAGTGT | |||||
Cytochrome C Oxidase Subunit VIIIA (COX8A) | NM_174024 | Oxidative Phosphorylation | Fwd: TTTGACTTCGCGACCTTGG | 60 | This study |
Rev: TTACGGCACGGAGTAGACTG | |||||
Solute Carrier Family 25 Member 39 (SLC25A39) | NM_001075415 | Mitochondrial substrate/solute carrier | Fwd: AGCTAATGCCTCCCTCCAGA | 54 | This study |
Rev: GGCACTTCCATTTGGCGTAG | |||||
Pyruvate Kinase M2 (PKM2) | NM_001205727 | Glycolysis, Gluconeogenesis | Fwd: TGTCACCCATTACCAGCGAC | 130 | This study |
Rev: TATCTGGCCACCTGATGTGC | |||||
SUZ12 polycomb repressive complex 2 subunit (SUZ12) | NM_001205587 | Endogenous control | Fwd: CATCCAAAAGGTGCTAGGATAGATG | 160 | [62] |
Rev: TGGGCCTGCACACAAGAATG |