Table 1 Primer pair sequences designed for validation of differentially expressed genes in CK30 and T30 treatment P. digitatum using Real-time Fluorescence Quantitative PCR (RTFQ-PCR)
Gene ID | Gene name | Primer sequence (5′-3′, forward/reverse) |
|---|---|---|
Unigene6144 | Actin | TGCGCTGAACCGAACTGCCG TCGGGAGCCTCGAAGCGCTC |
Unigene8313 | ERG7 | GCGCTGGCGATTGGTCGATG |
CAGGCCCAGTTTCCGGGCTCC | ||
Unigene8539 | ERG11 | CCATCGACCTCGTCCCCGCC |
TCGCGCTTGCGGTTTTGGGG | ||
Unigene6797 | ERG6 | CGCGTGATGCCGCCTTCAAC |
TGAGCCTTGCGGGCCTCACG | ||
Unigene5828 | ERG3 | CAGGCCATGGCCGCAATGCC |
GGTGCAGGCCACGGTGGATCC | ||
Unigene8125 | ERG5 | TCTCGCCATTGGCGGATGCG |
TCTCGCCATTGGCGGATGCG |