Fig. 4
From: A strategy to identify housekeeping genes suitable for analysis in breast cancer diseases
Scatter plot of gene expression measured by real-time PCR and read counting in RNA-seq data. a Expression pattern of nHKG candidates and tHKGs in breast cell lines obtained by qRT-PCR across eight cell lines. Boxes delimit lower and upper quartiles while the vertical lines within the boxes indicate median expression values. Lateral whiskers provide lower and higher values as left and right ticks, respectively. The x-axis gives the mean of 1/CT for each gene. Results are representative of three independent experiments. b Correlation between qRT-PCR and read count from RNA-seq data for each gene across eight breast cell lines (r = 0963). The y-axis represents the average of CVs for each gene over eight cell lines