Fig. 8

H3K27me3 levels on several differentially expressed genes by ChIP-seq and their qPCR verification. a The H3K27me3 modification profiles were shown on some enriched regions of CD4, ZNF200, GHR, TPM1, TPM2, TPM3, cast and IL10 genes. The structures of genes are displayed below the H3K27me3 profiles, in which black boxes represent exons and arrows represent the transcriptional direction of genes. The red vertical bars represent selected enriched regions. b Real-time qPCR data showed H3K27me3 enrichment on selected enriched regions of CD4 and IL10 genes for two groups of cattle. Five sites of CD4 validated by real-time PCR are corresponding to sites 1–5 of ChIP-seq in Fig. 8a1, while one site of IL10 validated by qPCR is same as that in ChIP-seq of Fig. 8a8. Negative controls used were GAPDH_P1 and 18 s rRNA_P1. c Real-time RT-qPCR were performed in two groups of cattle for validation of CD4 and IL10 expression (GAPDH, 18 s rRNA and beta-actin were used as housekeeping genes). The individual with the lowest corrected Ct value was selected as a control sample to calculate relative expression of genes. DGE indicates the results of digital gene expression of CD4 and IL10 (for TPM)