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Table 5 Primer sequences used for real-time PCR with amplicon size and annealing temperature. All primer sets had R2 > 0.98 and efficiency percentage between 90 % and 110 %

From: Determining the mode of action of anti-mycobacterial C17 diyne natural products using expression profiling: evidence for fatty acid biosynthesis inhibition

Gene

Forward primer (5′-3′)

Reverse primer (5′-3′)

Product size (bp)

Annealing (°C)

alkB (MEMEG 1839)

GCCTACATCCCGTTCCAGT

AGCGAGTCCTTCTTGTGTCC

191

58

ectB (MSMEG 3900)

AGGATTTTTCCGTGTTGCTC

TTGTTGTGCCCGTAGTTCAG

214

58

katG (MSMEG 3461)

GCCACCCAGGAAGAGACC

GCAGGTTGACGAAGAAGTCC

265

58

mmpl5 (MSMEG 1382)

CGAATCTGGCTACCTGTGCT

GTGGCGGTCCTCTCTTCTTT

365

58

MSMEG 3359

CACCGACATACACTGCCAAC

GAACCACGCCTTCTCCTG

310

58

MSMEG 3805

GGGGAGCCATTCTCAACG

TGTGTTCCTCGGGCAGTTC

225

59

pcaC (MSMEG 6370)

CGAGACCGAGCAGCGACT

CGGGAACGGCATCTTCAC

250

59

gidB (MSMEG 6940) 1

ATGCCAGGGTGGTGAGAT

CGTGAAACATTCGGCTTCT

270

61

MSMEG 3496

TATGAGCGTGGTGGTCCTG

GCGGTCGTTGTAGTTGGTCT

229

61

MSMEG 3584 a

TGTCGGAGTTGTTGATGGTC

CTGTCGGTGTTCTCGTTCAG

241

61

MSMEG 5570 a

CACCGAGAAAGAACTGAGCA

GCAACTATTCCCACACAACCT

173

60

  1. a Genes used as normalizer gene