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Fig. 5 | BMC Genomics

Fig. 5

From: The Rat microRNA body atlas; Evaluation of the microRNA content of rat organs through deep sequencing and characterization of pancreas enriched miRNAs as biomarkers of pancreatic toxicity in the rat and dog

Fig. 5

Correlation of qPCR analysis of tissue specific and/or enriched miRNAs. Tissue specific and/or enriched miRNAs were chosen according to tissues that lack or require additional biomarkers of organ status and were measured in 21 tissues from 3 female rats by qPCR. The qPCR data was compared to the sequencing raw counts for each miRNA. A perfect correlation is equal to − 1 because the Ct value decreases as sequencing abundance increases. Twenty seven of the thirty nine miRNAs tested correlated very well to the sequencing data with a correlation of − 0.8 to − 1.0, 8 miRNAs displayed a correlation of − 0.79 to − 0.57 and 4 miRNAs displayed poorer correlations from − 0.33 to − 0.22. MiRs-196c-5p and 206-5p were not amplified by qPCR and, therefore, did not display tissue specificity or enrichment by qPCR

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BMC Genomics

ISSN: 1471-2164

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