Fig. 6

POC Compound Y miRNA safety biomarker panel assessment. a Serum levels of ALT and AST in animals that received Compound Y or vehicle control. Serum was collected on Days 1, 7 and 14 at pre-dose and 24 h post-dose. The colored boxes on the right correspond to the individual dog identification numbers. b Panel of candidate safety miRNA biomarkers utilized in the Compound Y POC study. Q-RT-PCR ΔCt values (y-axis) line plot per animal for duration of Day 1, 7, and 14 treatment samples tested highlights the elevation of both liver enriched miRNAs (miR-122 and miR-885) and ubiquitously expressed miR-193 in the 2 dogs with elevated ALT and AST. Non-liver enriched miRNAs were not elevated or were found to be in the noise or below the lower limit of Q-RT-PCR detection