Sea lampreys elicit strong transcriptomic responses in the lake trout liver during parasitism

BMC Genomics

Table 9 Results of QPCR analysis of genes appearing in the top 25 up and down-regulated gene lists for parasitized and nonparasitized lean and siscowet lake trout (From Tables 5, 6, 7 and 8). Contigs provided in Additional file 2

			QPCR		^aRNA-seq
	Gene name	Contig #	Fold +/−	p value	Fold +/−
Lean	interleukin-18-binding protein	comp44889	26.4	0.004	9.51
	fibulin 4 precursor	comp81242	5.3	0.001	5.10
	glucose-6-phosphate isomerase	comp7122	3.2	0.004	2.36
	6-phosphogluconate dehydrogenase, decarboxylating	comp4673	3.4	0.005	2.77
	ornithine decarboxylase	comp4006	12.5	0.032	6.68
	cyclic AMP-dependent transcription factor ATF-3	comp224599	42.8	0.091	19.03
	indoleamine 2,3-dioxygenase 2	^bcomp228271	−5.2	0.007
	indoleamine 2,3-dioxygenase 2	comp275449	−5.8	0.002	−6.87
	adenylosuccinate synthetase isozyme 1 A	comp122752	−4.1	0.001	−4.53
	UDP-glucuronosyltransferase 2A2	comp11363	−3.8	0.001	−4.53
	phosphotriesterase related	comp61354	−9.0	0.010	−14.52
	aspartoacylase	comp190057	−4.4	0.001	−4.99
Siscowet	growth arrest and DNA damage inducible protein	comp8599	7.5	0.012	7.01
	chemokine-like receptor 1	comp22172	3.3	0.038	6.59
	interleukin-18-binding protein	comp44889	6.8	0.054	7.46
	dual specificity protein phosphatase 2	comp204068	59.1	0.070	16.34
	ubiquitin carboxyl-terminal hydrolase 24	comp72729	2.9	0.004	4.96
	phosphotriesterase related	comp61354	−9.7	0.003	−7.26
	DNA damage-regulated autophagy modulator protein 2	comp92592	−7.0	0.008	−5.86
	indoleamine 2,3-dioxygenase 2	comp275449	−3.0	0.019	−5.62
	indoleamine 2,3-dioxygenase 2	comp228271	−5.7	0.001	−8.34
	GTP-binding protein Rhes	comp55581	−8.3	0.003	−11.39
	C-C motif chemokine 19	comp242068	−7.6	0.003	−11.47

^awhen present multiple times or in both DESeq2 and edgeR analyses, is the average fold change of all occurrences
^bcontig, comp228271, was not represented in parasitized leans but was still tested by QPCR

ISSN: 1471-2164