Fig. 5

RT-qPCR validation of time-course RNA-seq data using six selected lincRNAs differentially expressed over time. 18S rRNA and elongation factor 1-α (ef1α) were used as the reference genes. The relative expression levels of lincRNAs at each time point were calculated relative to calibrator (control sample; 0 hpi). Error bars represent the range of relative expression (fold change) calculated by 2^{-(ΔΔCt±SD)}. Two biological replicates were used in triplicate