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Table 1 Proteins with a significantly lower or higher expression in treatment and control groups

From: A novel approach to assessing bisphenol-A hazards using an in vitro model system

Symbol Protein description gi no. MASCOT score* Relative intensitya (normalized)
Control 0.0001 0.01 1 100
Energy metabolism (46 %)
 ATP5H ATP synthase subunit d, mitochondrial gi|21313679 58 1A 0.99 ± 0.15A 0.93 ± 0.12A 0.63 ± 0.01A,B 0.36 ± 0.12B
 ATP5O ATP synthase subunit O, mitochondrial gi|20070412 69 1A 0.97 ± 0.19A 0.68 ± 0.14A,B 0.62 ± 0.08A,B 0.44 ± 0.07B
HADHA Trifunctional enzyme subunit alpha, mitochondrial gi|33859811 66 1A 0.71 ± 0.01A,B 0.41 ± 0.03B,C 0.40 ± 0.04B,C 0.31 ± 0.10C
COX6A1 Cytochrome c oxidase subunit 6A1, mitochondrial gi|1352173 49 1A 0.68 ± 0.19A,B 0.28 ± 0.07B,C 0.23 ± 0.06B,C 0.17 ± 0.01C
PGAM2 Phosphoglycerate mutase 2 gi|9256624 53 1A 0.99 ± 0.13A 0.76 ± 0.13A,B 0.72 ± 0.08A,B 0.34 ± 0.07B
OXCT2A Succinyl-CoA:3-ketoacid coenzyme A transferase 2A, mitochondrial gi|81881929 36 1A 0.71 ± 0.16A,B 0.67 ± 0.07A,B 0.65 ± 0.04A,B 0.43 ± 0.09B
ISOC2A Isochorismatase domain-containing protein 2A, mitochondrial gi|197333728 73 1A 0.83 ± 0.19A,B 0.79 ± 0.08A,B 0.75 ± 0.10A,B 0.34 ± 0.12B
GAPDH Glyceraldehyde-3-phosphate dehydrogenase, testis-specific gi|2494630 37 1A 1.09 ± 0.14A 1.11 ± 0.25A,B 1.12 ± 0.31A,B 2.17 ± 0.28B
 AK2 Adenylate kinase 2, mitochondrial isoform gi|34328230 70 1A 1.13 ± 0.18 A,B 1.19 ± 0.27A,B 1.37 ± 0.11A,B 2.05 ± 0.14B
 SDHB Succinate dehydrogenase Ip subunit gi|34328286 63 1A 2.59 ± 0.66A,B 3.02 ± 0.29A,B 3.32 ± 0.56B 3.27 ± 0.38B
UQCRFS1 Cytochrome b-c1 complex subunit Rieske, mitochondrial gi|13385168 62 1A 1.56 ± 0.17A,B 1.62 ± 0.40A,B 1.72 ± 0.32A,B 2.16 ± 0.01B
Cytoskeletal/structural proteins (25 %)
ROPN1 Ropporin-1 gi|74227586 202 1A 0.88 ± 0.12A 0.78 ± 0.10A,B 0.71 ± 0.10A,B 0.49 ± 0.01B
ACTB Actin, cytoplasmic 1 gi|6671509 51 1A 0.93 ± 0.17A 0.92 ± 0.16A 0.49 ± 0.04B 0.47 ± 0.04B
 FABP9 Fatty acid-binding protein 9 gi|166897974 68 1A 0.94 ± 0.05A 0.86 ± 0.13A 0.68 ± 0.03A,B 0.47 ± 0.07B
 ODF2 Outer dense fiber protein 2 gi|2290719 57 1A 0.68 ± 0.14A,B 0.62 ± 0.19A,B 0.59 ± 0.16A,B 0.13 ± 0.01B
 PMP20 Peroxisomal membrane protein 20 gi|6746357 79 1A 1.06 ± 0.05A 1.10 ± 0.06A,B 1.17 ± 0.22A,B 2.11 ± 0.01B
ASRGL1 Isoaspartyl peptidase/L-asparaginase gi|81875980 52 1A 1.38 ± 0.12A,B 2.09 ± 0.87A,B 2.79 ± 0.42A,B 3.23 ± 0.36B
Fertility Related Proteins (17 %)
 PHB Prohibitin gi|6679299 68 1A 0.91 ± 0.12A 0.81 ± 0.20A,B 0.75 ± 0.12A,B 0.43 ± 0.05B
PPP1CA Serine/threonine-protein phosphatase PP1-alpha catalytic subunit gi|49065812 50 1A 0.94 ± 0.16A 0.60 ± 0.20A,B 0.47 ± 0.07A,B 0.36 ± 0.05B
DNAL1 Dynein light chain 1, axonemal gi|164607162 67 1A 0.74 ± 0.01A,B 0.65 ± 0.14 A,B 0.64 ± 0.23A,B 0.49 ± 0.13B
 SPA17 Sperm surface protein Sp17 gi|6755614 58 1A 1.11 ± 0.21A 1.39 ± 0.81A 1.59 ± 0.05A,B 2.08 ± 0.17B
Stress response proteins/ROS metabolism (12 %)
GSTM5 Glutathione S-transferase Mu 5 gi|6754086 70 1A 0.92 ± 0.26A,B 0.67 ± 0.08A,B 0.48 ± 0.09B 0.32 ± 0.08B
 GPX4 Glutathione peroxidase gi|3075477 65 1A 1.71 ± 0.08A,B 1.87 ± 0.19B 2.24 ± 0.24B 2.25 ± 0.15B
 PRDX5 Peroxiredoxin-5, mitochondrial gi|6755114 73 1A 1.28 ± 0.17A,B 1.30 ± 0.15A,B 2.02 ± 0.11B 2.35 ± 0.09B
  1. *MASCOT score is −10 log (P), where P is the probability that the observed match is a random event. Individual scores > 30 indicate identity or extensive homology (p < 0.05). aRelative spots intensity in control and BPA-treated (μM) spermatozoa. Data are presented as mean ± SEM (3 replicates). Values with different superscript characters (A,B,C) indicate significant differences between the control and treatment groups as determined by one-way ANOVA (P < 0.05)