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Table 1 Proteins with a significantly lower or higher expression in treatment and control groups

From: A novel approach to assessing bisphenol-A hazards using an in vitro model system

Symbol

Protein description

gi no.

MASCOT score*

Relative intensitya (normalized)

Control

0.0001

0.01

1

100

Energy metabolism (46 %)

 ATP5H

ATP synthase subunit d, mitochondrial

gi|21313679

58

1A

0.99 ± 0.15A

0.93 ± 0.12A

0.63 ± 0.01A,B

0.36 ± 0.12B

 ATP5O

ATP synthase subunit O, mitochondrial

gi|20070412

69

1A

0.97 ± 0.19A

0.68 ± 0.14A,B

0.62 ± 0.08A,B

0.44 ± 0.07B

HADHA

Trifunctional enzyme subunit alpha, mitochondrial

gi|33859811

66

1A

0.71 ± 0.01A,B

0.41 ± 0.03B,C

0.40 ± 0.04B,C

0.31 ± 0.10C

COX6A1

Cytochrome c oxidase subunit 6A1, mitochondrial

gi|1352173

49

1A

0.68 ± 0.19A,B

0.28 ± 0.07B,C

0.23 ± 0.06B,C

0.17 ± 0.01C

PGAM2

Phosphoglycerate mutase 2

gi|9256624

53

1A

0.99 ± 0.13A

0.76 ± 0.13A,B

0.72 ± 0.08A,B

0.34 ± 0.07B

OXCT2A

Succinyl-CoA:3-ketoacid coenzyme A transferase 2A, mitochondrial

gi|81881929

36

1A

0.71 ± 0.16A,B

0.67 ± 0.07A,B

0.65 ± 0.04A,B

0.43 ± 0.09B

ISOC2A

Isochorismatase domain-containing protein 2A, mitochondrial

gi|197333728

73

1A

0.83 ± 0.19A,B

0.79 ± 0.08A,B

0.75 ± 0.10A,B

0.34 ± 0.12B

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase, testis-specific

gi|2494630

37

1A

1.09 ± 0.14A

1.11 ± 0.25A,B

1.12 ± 0.31A,B

2.17 ± 0.28B

 AK2

Adenylate kinase 2, mitochondrial isoform

gi|34328230

70

1A

1.13 ± 0.18 A,B

1.19 ± 0.27A,B

1.37 ± 0.11A,B

2.05 ± 0.14B

 SDHB

Succinate dehydrogenase Ip subunit

gi|34328286

63

1A

2.59 ± 0.66A,B

3.02 ± 0.29A,B

3.32 ± 0.56B

3.27 ± 0.38B

UQCRFS1

Cytochrome b-c1 complex subunit Rieske, mitochondrial

gi|13385168

62

1A

1.56 ± 0.17A,B

1.62 ± 0.40A,B

1.72 ± 0.32A,B

2.16 ± 0.01B

Cytoskeletal/structural proteins (25 %)

ROPN1

Ropporin-1

gi|74227586

202

1A

0.88 ± 0.12A

0.78 ± 0.10A,B

0.71 ± 0.10A,B

0.49 ± 0.01B

ACTB

Actin, cytoplasmic 1

gi|6671509

51

1A

0.93 ± 0.17A

0.92 ± 0.16A

0.49 ± 0.04B

0.47 ± 0.04B

 FABP9

Fatty acid-binding protein 9

gi|166897974

68

1A

0.94 ± 0.05A

0.86 ± 0.13A

0.68 ± 0.03A,B

0.47 ± 0.07B

 ODF2

Outer dense fiber protein 2

gi|2290719

57

1A

0.68 ± 0.14A,B

0.62 ± 0.19A,B

0.59 ± 0.16A,B

0.13 ± 0.01B

 PMP20

Peroxisomal membrane protein 20

gi|6746357

79

1A

1.06 ± 0.05A

1.10 ± 0.06A,B

1.17 ± 0.22A,B

2.11 ± 0.01B

ASRGL1

Isoaspartyl peptidase/L-asparaginase

gi|81875980

52

1A

1.38 ± 0.12A,B

2.09 ± 0.87A,B

2.79 ± 0.42A,B

3.23 ± 0.36B

Fertility Related Proteins (17 %)

 PHB

Prohibitin

gi|6679299

68

1A

0.91 ± 0.12A

0.81 ± 0.20A,B

0.75 ± 0.12A,B

0.43 ± 0.05B

PPP1CA

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

gi|49065812

50

1A

0.94 ± 0.16A

0.60 ± 0.20A,B

0.47 ± 0.07A,B

0.36 ± 0.05B

DNAL1

Dynein light chain 1, axonemal

gi|164607162

67

1A

0.74 ± 0.01A,B

0.65 ± 0.14 A,B

0.64 ± 0.23A,B

0.49 ± 0.13B

 SPA17

Sperm surface protein Sp17

gi|6755614

58

1A

1.11 ± 0.21A

1.39 ± 0.81A

1.59 ± 0.05A,B

2.08 ± 0.17B

Stress response proteins/ROS metabolism (12 %)

GSTM5

Glutathione S-transferase Mu 5

gi|6754086

70

1A

0.92 ± 0.26A,B

0.67 ± 0.08A,B

0.48 ± 0.09B

0.32 ± 0.08B

 GPX4

Glutathione peroxidase

gi|3075477

65

1A

1.71 ± 0.08A,B

1.87 ± 0.19B

2.24 ± 0.24B

2.25 ± 0.15B

 PRDX5

Peroxiredoxin-5, mitochondrial

gi|6755114

73

1A

1.28 ± 0.17A,B

1.30 ± 0.15A,B

2.02 ± 0.11B

2.35 ± 0.09B

  1. *MASCOT score is −10 log (P), where P is the probability that the observed match is a random event. Individual scores > 30 indicate identity or extensive homology (p < 0.05). aRelative spots intensity in control and BPA-treated (μM) spermatozoa. Data are presented as mean ± SEM (3 replicates). Values with different superscript characters (A,B,C) indicate significant differences between the control and treatment groups as determined by one-way ANOVA (P < 0.05)