In silico analyses of mitochondrial ORFans in freshwater mussels (Bivalvia: Unionoida) provide a framework for future studies of their origin and function

Table 2 p-distances (p-D) and standard error (SE) values for mitochondrial M-orfs, F-orfs, cox1 and atp8 in freshwater mussel subfamilies

Subfamily	Gene (N)	Nucleotide		Amino acid		p
Subfamily	Gene (N)	p-D	SE	p-D	SE
Unioninae	F-orf (3)	0.355	0.023	0.467	0.047	1.000
	F-cox1 (2)	0.103	0.007	0.014	0.005	1.000
	F-atp8 (2)	0.300	0.011	0.333	0.015	1.000
	M-orf (2)	0.350	0.018	0.502	0.034	1.000
	M-cox1 (2)	0.165	0.010	0.094	0.012	1.000
	M-atp8 (2)	0.250	0.010	0.267	0.013	1.000
Gonideinae	F-orf (2)	0.469	0.033	0.692	0.058	1.000
	F-cox1 (2)	0.132	0.008	0.033	0.008	1.000
	F-atp8 (2)	0.400	0.025	0.222	0.010	1.000
	M-orf (2)	0.384	0.025	0.552	0.044	1.000
	M-cox1 (2)	0.175	0.009	0.130	0.015	1.000
	M-atp8 (2)	0.301	0.019	0.421	0.039	1.000
Ambleminae	F-orf (3)	0.351	0.024	0.508	0.041	1.000
	F-cox1 (2)	0.128	0.009	0.033	0.007	1.000
	F-atp8 (2)	0.278	0.018	0.370	0.031	1.000
	M-orf (2)	0.421	0.027	0.687	0.047	1.000
	M-cox1 (2)	0.179	0.010	0.145	0.015	1.000
	M-atp8 (2)	0.211	0.012	0.233	0.017	1.000
Margaritiferinae	F-orf (2)	0.393	0.029	0.705	0.050	1.000
Margaritiferinae	F-cox1 (2)	0.164	0.009	0.068	0.009	1.000

Note: N number of sequences used. The probability of rejecting the null hypothesis of strict-neutrality (d _N = d _S) in favor of the alternative hypothesis (d _N > d _S) (in the p column) is shown. d _S and d _N are the numbers of synonymous and nonsynonymous substitutions per site, respectively

ISSN: 1471-2164