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Fig. 3 | BMC Genomics

Fig. 3

From: Transcriptomic analysis of the autophagy machinery in crustaceans

Fig. 3

Identification of an M. rosenbergii Atg8. a A schematic annotation of M. rosenbergii Atg8 (MroAtg8) and the alignment of crustacean Atg8 proteins with Atg8/LC3 of other species from different phyla. Black shading indicates conserved amino acids while grey shading indicates similar amino acids. Blue boxes indicate the basic residues which are previously known to be critical for electrostatic interaction of LC3 with other proteins. Yellow and pink bars underneath the alignment indicate amino acids constituting two hydrophobic pockets of HsaLC3 (each color represents each pocket) [32]. Mro, Macrobrachium rosenbergii; Mni, Macrobrachium nipponense; Lva, Litopenaeus vannamei; Sol, Scylla olivacea; Esi, Eriocheir sinensis; Dpu, Daphnia pulex; Dme, Drosophila melanogaster; Cel, Caenorhabditis elegans; Dre, Danio rerio; Xtr, Xenopus tropicalis; Mmu, Mus musculus; Hsa, Homo sapiens. b Predicted tertiary structures of HsaLC3 (a) and MroAtg8 (b) and superimposition of HsaLC3 and MroAtg8 (c). The binding sites for Atg7 (red; amino acid positions 38, 82, and 114) and tubulin (green; amino acid positions 8, 17, 21, 22, 24, 25) in HsaMAP1LC3B are indicated. c Predicted MroAtg8 tertiary structure with hydrophobic surface. Residues which were predicted to form two hydrophobic pockets in MroAtg8 are colored in yellow and pink

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