Fig. 7
From: Transcriptomic analysis of the autophagy machinery in crustaceans
Immunoperoxidase staining of Atg marker proteins in the ovary of M. rosenbergii. Late vitellogenic oocyte (Oc4) with H&E stain (A). Immunolocalization of Atg6/BECN1 (B), Atg8/LC3 (C), p62/SQSTM1 (D), and Lamp-1 (E) in the Oc4 of ovarian sections. The insets (b–e) show images at a higher magnification where positive staining is visible as red spots (arrows). F Negative control. Scale bars: 25 μm (A and F) and 10 μm (B–E). (N, nucleus; Yg, yolk granules; Li, lipid droplets; Mb, cell membrane)