Fig. 3

Expression analysis of the drought-responsive miRNA precursor miR-171f and its putative target genes (Os03g0828701-00, Os12g0571900-01, Os09g0555600-01 and Os05g0417100-01) in various plant tissues at different developmental stages. Rice seeds were germinated and grown on MS (Murashige and Skoog) medium in the dark for 3 d (3 DAG, day after germination) and then in the light for 1 d at 28 °C (4 DAG). Seedlings were then transplanted into soil pots, and grown in the greenhouse for 10 d, 15 d, 1 month and 2 month until meiosis (meiosis), just prior to heading (before heading, BH) and right after heading (after heading, AH). qRT-PCR analyses of each gene were performed with the indicated tissues at the different developmental stages. Rice Ubi1 (AK121590) was used as an internal control. C, coleoptiles; R, roots; L, leaves; FL, flag leaves; F, flowers (panicles). Bar indicated as mean values ± SD (standard deviation) of three independent experiments