Fig. 6

ER stress and amino acid starvation induce a common alternative splicing signature. a Venn diagrams demonstrating the overlap between the AS events occurring during thapsigargin treatment and nutrient starvation. The overlap p value was calculated using a hypergeometric test. b Validation of AS events for Hnrnpd, Ogt, Srsf7, Ivns1abp, Hnrpdl. Gene schematics highlighting the AS events along with approximate location of the premature termination codon (PTC), along with PSI value plots for each event. Semi-quantitative RT-PCR validations for each event are shown, with the spliced PCR product labeled by an arrow. IGV plots of RNA-seq read for each AS event and adjacent sequence reads, with AS events highlighted accordingly