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Fig. 8 | BMC Genomics

Fig. 8

From: The transcriptional and splicing landscape of intestinal organoids undergoing nutrient starvation or endoplasmic reticulum stress

Fig. 8

Validation of various alternative splicing events and in silico prediction of alternative ATG usage in splice variants induced upon cellular stress. a-b Six selected AS events induced upon thapsigargin treatment (Casp4, Slc35b1, Ufdl1) (a) and nutrient starvation (Frrs, Nnt, Nt5c3) (b). The type of AS event is indicated beside the gene name in parenthesis (S – skipping, Alt3 – alternative 3’, Alt5 – alternative 5’, C3 – complex type 3). Plots depicting the percent spliced-in (PSI) values for the AS events are shown. c-d Gene structures of full-length Casp4, Slc35b1, Nt5c3, Tinag, and Ufd1l using the canonical translation start sites (cATG) and their predicted splice variants induced by cellular stress (AS event highlighted in red). The alternative ATG (aATG) utilized by these variants were previously annotated by Aceview. The different protein domains encoded by the full-length and splice variants were analyzed using Simple Modular Architecture Research Tool (SMART) (c) and changes in protein localization were analyzed using PSORT (d)

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